BBAPro Magazine annual cover!Mapping of immune molecules at different stages of COVID-19

2022-07-03 0 By

COVID-19 continues to spread globally, posing a serious threat to human health and socio-economic development.According to Worldometer, as of February 14, 2022, there were 153,001 new confirmed cases and 6,383 new deaths in a single day worldwide.Effective response to COVID-19 requires not only prevention and treatment to improve survival rates, but also early intervention strategies to deal with sequelae of patients. Currently, the molecular mechanism of sequelae in COVID-19 patients has not been clearly defined.Recently, the study of precision medical more groups of Peking University health science center, professor Huang Chaolan team and Gao Fu academician of Chinese Academy of Sciences team, researcher at the Chinese centers for disease control and prevention virus disease is liujun team to carry out the cooperation study, based on the spanning “asymptomatic, light in the disease, severe, rehabilitation” of the four COVID queue – 19 patients, through the integration of proteomics data with clinical data analysis,It has been found that the immune function of patients with COVID-19 has a “multi-stage” process of asymptomatic activation, early suppression of disease, and abnormal activation at the later stage. The functional change of neutrophils may be the turning point of immune dysfunction.At the same time, immune dysfunction, cholesterol metabolism disorders and impaired cardiac function are present throughout the onset and progression of COVID-19.The findings have implications for the development of early intervention strategies to improve patient outcomes.The study was published on the topic of Immune response patterns across the asymptomatic,symptomatic and convalescent periods of COVID-19 in BBA-ProteinsAnd Proteomics, and was featured in the 2022 cover article of the journal.Graph:Immune dysfunction, impaired cholesterol metabolism and impaired heart muscle function run through different stages of COVID-19We performed diA-PASEF quantitative proteomic analysis of serum and urine samples from COVID-19 patients at various stages of disease progression.The results showed that the immune response was activated in asymptomatic patients compared with healthy controls, but was disordered to varying degrees in mild and severe patients. The turning point of the immune response was the change of neutrophil function.In addition, the recovered patients showed significant immune suppression, which lasted up to 12 months after recovery.This study suggests that long-term disorders of immune response, cholesterol metabolism and cardiovascular function may be the key causes of potential sequelae, and the relevant research results comprehensively reveal the immune molecular map of different stages of COVID-19, which will contribute to the further exploration of early intervention strategies to effectively improve the sequelae of complex diseases in the future.This study, based on serum and urine proteomics data with ultra-high identification depth and accuracy, provides reliable and important molecular basis and mechanism information for the comprehensive exploration of prognosis assessment in PATIENTS with COVID-19.Under the leadership of Professor Huang Chaolan, the Multi-omics Center has made many important achievements in COVID-19 research based on the deep integration of clinical, cutting-edge technologies and basic disciplines.Previously, the multi-omics Center team led by Director Huang Zhaolan and the multidisciplinary team led by Academician Gao Fu worked closely together and found that the early novel coronavirus infection was mainly immunosuppressed or had a “two-stage” mechanism. By mapping the glycosylation map of novel coronavirus spike protein, the new law of “O-follow-up” glycosylation was revealed for the first time.In addition, consistent with the results of this study, Huang Chaolan’s team also carried out a collaborative study with Professor Zheng Min’s team from the First Affiliated Hospital of Zhejiang University, which for the first time focused on changes in serum protein expression in patients who had recovered from COVID-19, and proposed that patients who had recovered would still suffer from cholesterol metabolism disorder and myocardial damage one month later.Under the leadership of Professor Huang Chao-lan, the multi-omics Center team has always been committed to developing innovative approaches to mass spectrometry and proteomics, exploring and revealing the unknown areas of life sciences, and achieving real outcomes that contribute to life sciences and human health from the origins of significant scientific and clinical problems.Gao Fu academician of Chinese Academy of Sciences, China CDC virus disease liujun by researchers and more accurate medical group study Peking University health science center director professor Huang Chaolan corresponding author together for the paper, study of precision medical more groups of Peking University health science center Chen Yangfu researcher Dr., nan, Dr Zhang jie by the centers for disease control and prevention virus disease in China,Jiangtao Guo, Multi-omics Research Center for Precision Medicine, Peking University Health Science Center, and Shaobo Dong, Macheng Center for Disease Control and Prevention, Hubei Province, co-authored the paper.The original link: reference 1. Tian,W. M. et al. Immune suppression in the early stage of COVID-19 disease. Nat Commun 11, Doi: 10.1038 / s41467-020-19706-9 (2020). 2. Tian,W. M. et al. O-glycosylation pattern of the SARS-CoV-2 spike protein reveals an “O-Follow-N” rule. Cell Res 31, 1123-1125, doi: 10.1038 / s41422-021-00545-2 (2021). 3. Chen,Y. et al. Proteomic Analysis Identifies Prolonged Disturbances in Pathways Related toCholesterol Metabolism and Myocardium Function in the COVID-19 Recovery Stage. J Proteome Res 20, 3463-3474, C00054 doi: 10.1021 / acs. Jproteome. 1 (2021).